|
|
|
|
| LEADER |
01765 am a22003013u 4500 |
| 001 |
123502 |
| 042 |
|
|
|a dc
|
| 100 |
1 |
0 |
|a Jain, Piyush Kumar
|e author
|
| 100 |
1 |
0 |
|a Massachusetts Institute of Technology. Institute for Medical Engineering & Science
|e contributor
|
| 100 |
1 |
0 |
|a Massachusetts Institute of Technology. Department of Biological Engineering
|e contributor
|
| 100 |
1 |
0 |
|a Massachusetts Institute of Technology. Department of Chemical Engineering
|e contributor
|
| 100 |
1 |
0 |
|a Broad Institute of MIT and Harvard
|e contributor
|
| 700 |
1 |
0 |
|a Lo, Justin H.
|e author
|
| 700 |
1 |
0 |
|a Rananaware, Santosh
|e author
|
| 700 |
1 |
0 |
|a Downing, Marco
|e author
|
| 700 |
1 |
0 |
|a Panda, Apekshya
|e author
|
| 700 |
1 |
0 |
|a Tai, Michelle
|e author
|
| 700 |
1 |
0 |
|a Raghavan, Srivatsan
|e author
|
| 700 |
1 |
0 |
|a Fleming, Heather
|e author
|
| 700 |
1 |
0 |
|a Bhatia, Sangeeta N
|e author
|
| 245 |
0 |
0 |
|a Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes
|
| 260 |
|
|
|b Royal Society of Chemistry (RSC),
|c 2020-01-21T20:28:58Z.
|
| 856 |
|
|
|z Get fulltext
|u https://hdl.handle.net/1721.1/123502
|
| 520 |
|
|
|a There is a critical need for the development of safe and efficient delivery technologies for CRISPR/Cas9 to advance translation of genome editing to the clinic. Non-viral methods that are simple, efficient, and completely based on biologically-derived materials could offer such potential. Here we report a simple and modular tandem peptide-based nanocomplex system with cell-targeting capacity that efficiently combines guide RNA (sgRNA) with Cas9 protein, and facilitates internalization of sgRNA/Cas9 ribonucleoprotein complexes to yield robust genome editing across multiple cell lines
|
| 546 |
|
|
|a en
|
| 690 |
|
|
|a General Materials Science
|
| 655 |
7 |
|
|a Article
|
| 773 |
|
|
|t Nanoscale
|
