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|a dc
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|a Jain, Piyush Kumar
|e author
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|a Massachusetts Institute of Technology. Institute for Medical Engineering & Science
|e contributor
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|a Massachusetts Institute of Technology. Department of Biological Engineering
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|a Massachusetts Institute of Technology. Department of Chemical Engineering
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|a Broad Institute of MIT and Harvard
|e contributor
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|a Lo, Justin H.
|e author
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|a Rananaware, Santosh
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|a Downing, Marco
|e author
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|a Panda, Apekshya
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|a Tai, Michelle
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|a Raghavan, Srivatsan
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|a Fleming, Heather
|e author
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|a Bhatia, Sangeeta N
|e author
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|a Non-viral delivery of CRISPR/Cas9 complex using CRISPR-GPS nanocomplexes
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|b Royal Society of Chemistry (RSC),
|c 2020-01-21T20:28:58Z.
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|z Get fulltext
|u https://hdl.handle.net/1721.1/123502
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|a There is a critical need for the development of safe and efficient delivery technologies for CRISPR/Cas9 to advance translation of genome editing to the clinic. Non-viral methods that are simple, efficient, and completely based on biologically-derived materials could offer such potential. Here we report a simple and modular tandem peptide-based nanocomplex system with cell-targeting capacity that efficiently combines guide RNA (sgRNA) with Cas9 protein, and facilitates internalization of sgRNA/Cas9 ribonucleoprotein complexes to yield robust genome editing across multiple cell lines
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|a en
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|a General Materials Science
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|a Article
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|t Nanoscale
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