A CRISPR/Cas9 platform for MS2-labelling of single mRNA in live stem cells
The MS2 system is a powerful tool for investigating transcription dynamics at the single molecule directly in live cells. In the past, insertion of the RNA-labelling cassette at specific gene loci has been a major hurdle. Here, we present a CRISPR/Cas9-based approach to insert an MS2 cassette with s...
| Main Authors: | , , , , , |
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| Other Authors: | , |
| Format: | Article |
| Language: | English |
| Published: |
Elsevier BV,
2020-02-05T20:07:37Z.
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| Subjects: | |
| Online Access: | Get fulltext |