High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR-Cas9 in yeast
Construction and characterization of large genetic variant libraries is essential for understanding genome function, but remains challenging. Here, we introduce a Cas9-based approach for generating pools of mutants with defined genetic alterations (deletions, substitutions, and insertions) with an e...
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| Other Authors: | , |
| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC,
2020-04-23T15:13:32Z.
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| Subjects: | |
| Online Access: | Get fulltext |