High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR-Cas9 in yeast
Construction and characterization of large genetic variant libraries is essential for understanding genome function, but remains challenging. Here, we introduce a Cas9-based approach for generating pools of mutants with defined genetic alterations (deletions, substitutions, and insertions) with an e...
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| Other Authors: | , |
| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media LLC,
2020-04-23T15:13:32Z.
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| Subjects: | |
| Online Access: | Get fulltext |
| Summary: | Construction and characterization of large genetic variant libraries is essential for understanding genome function, but remains challenging. Here, we introduce a Cas9-based approach for generating pools of mutants with defined genetic alterations (deletions, substitutions, and insertions) with an efficiency of 80-100% in yeast, along with methods for tracking their fitness en masse. We demonstrate the utility of our approach by characterizing the DNA helicase SGS1 with small tiling deletion mutants that span the length of the protein and a series of point mutations against highly conserved residues in the protein. In addition, we created a genome-wide library targeting 315 poorly characterized small open reading frames (smORFs, <100 amino acids in length) scattered throughout the yeast genome, and assessed which are vital for growth under various environmental conditions. Our strategy allows fundamental biological questions to be investigated in a high-throughput manner with precision. United States. Defense Threat Reduction Agency (Grant HDTRA1-14-1-0006) |
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