Predictable and precise template-free CRISPR editing of pathogenic variants

Predictable and precise template-free CRISPR editing of pathogenic variants

Following Cas9 cleavage, DNA repair without a donor template is generally considered stochastic, heterogeneous and impractical beyond gene disruption. Here, we show that template-free Cas9 editing is predictable and capable of precise repair to a predicted genotype, enabling correction of disease-as...

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Main Authors: Shen, Max Walt (Author), Arbab, Mandana (Author), Hsu, Jonathan Yee-Ting (Author), Worstell, Daniel (Author), Culbertson, Sannie J. (Author), Krabbe, Olga (Author), Cassa, Christopher A. (Author), Liu, David R. (Author), Gifford, David K. (Author), Sherwood, Richard I. (Author)
Other Authors: Massachusetts Institute of Technology. Computational and Systems Biology Program (Contributor), Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory (Contributor), Broad Institute of MIT and Harvard (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science (Contributor)
Format: Article
Language:English
Published: Springer Nature, 2020-05-07T13:51:28Z.
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