RNA-guided DNA insertion with CRISPR-associated transposases

RNA-guided DNA insertion with CRISPR-associated transposases

CRISPR-Cas nucleases are powerful tools for manipulating nucleic acids; however, targeted insertion of DNA remains a challenge, as it requires host cell repair machinery. Here we characterize a CRISPR-associated transposase from cyanobacteria Scytonema hofmanni (ShCAST) that consists of Tn7-like tra...

Full description

Bibliographic Details
Main Authors: Strecker, Jonathan (Author), Ladha, Alim (Author), Gardner, Zachary (Author), Schmid-Burgk, Jonathan L. (Author), Makarova, Kira S. (Author), Koonin, Eugene V. (Author), Zhang, Feng (Author)
Other Authors: McGovern Institute for Brain Research at MIT (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor)
Format: Article
Language:English
Published: American Association for the Advancement of Science (AAAS), 2020-05-13T14:53:56Z.
Subjects:
Article
Online Access:Get fulltext

Internet

Get fulltext

Similar Items