Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1

Structural Basis for the Canonical and Non-canonical PAM Recognition by CRISPR-Cpf1

The RNA-guided Cpf1 (also known as Cas12a) nuclease associates with a CRISPR RNA (crRNA) and cleaves the double-stranded DNA target complementary to the crRNA guide. The two Cpf1 orthologs from Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium (LbCpf1) have been harnessed for eukaryotic gen...

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Bibliographic Details
Main Authors: Yamano, Takashi (Author), Zetsche, Bernd (Author), Ishitani, Ryuichiro (Author), Zhang, Feng (Author), Nishimasu, Hiroshi (Author), Nureki, Osamu (Author)
Other Authors: Broad Institute of MIT and Harvard (Contributor), McGovern Institute for Brain Research at MIT (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor)
Format: Article
Language:English
Published: Elsevier BV, 2020-05-27T12:52:30Z.
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