One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation

One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation

We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination...

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Bibliographic Details
Main Authors: Jacobsen, Johanne T. (Author), Mesin, Luka (Author), Markoulaki, Styliani (Author), Schiepers, Ariën (Author), Cavazzoni, Cecília B. (Author), Bousbaine, Djenet (Author), Jaenisch, Rudolf (Author), Victora, Gabriel D. (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor)
Format: Article
Language:English
Published: Rockefeller University Press, 2020-06-23T21:22:27Z.
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