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125966 |
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|a Jacobsen, Johanne T.
|e author
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|a Massachusetts Institute of Technology. Department of Biology
|e contributor
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|a Mesin, Luka
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|a Markoulaki, Styliani
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|a Schiepers, Ariën
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|a Cavazzoni, Cecília B.
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|a Bousbaine, Djenet
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|a Jaenisch, Rudolf
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|a Victora, Gabriel D.
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|a One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation
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|b Rockefeller University Press,
|c 2020-06-23T21:22:27Z.
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|z Get fulltext
|u https://hdl.handle.net/1721.1/125966
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|a We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR, and affinity maturation.
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|a National Institute of Allergy and Infectious Diseases (Grant R01AI119006)
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|a National Institute of Allergy and Infectious Diseases (Grant R21AI138020)
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|a Article
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|t Journal of Experimental Medicine
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