A Proteomics Approach to Profiling the Temporal Translational Response to Stress and Growth
To quantify dynamic protein synthesis rates, we developed MITNCAT, a method combining multiplexed isobaric mass tagging with pulsed SILAC (pSILAC)and bio-orthogonal non-canonical amino acid tagging (BONCAT)to label newly synthesized proteins with azidohomoalanine (Aha), thus enabling high temporal r...
Main Authors: | , , , , |
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Other Authors: | , , |
Format: | Article |
Language: | English |
Published: |
Elsevier BV,
2020-07-31T13:28:44Z.
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Subjects: | |
Online Access: | Get fulltext |