A Proteomics Approach to Profiling the Temporal Translational Response to Stress and Growth

A Proteomics Approach to Profiling the Temporal Translational Response to Stress and Growth

To quantify dynamic protein synthesis rates, we developed MITNCAT, a method combining multiplexed isobaric mass tagging with pulsed SILAC (pSILAC)and bio-orthogonal non-canonical amino acid tagging (BONCAT)to label newly synthesized proteins with azidohomoalanine (Aha), thus enabling high temporal r...

Full description

Bibliographic Details
Main Authors: Rothenberg, Daniel Abram (Author), Taliaferro, Jefferson Matthew (Author), Huber, Sabrina M (Author), Dedon, Peter C (Author), White, Forest M. (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Koch Institute for Integrative Cancer Research at MIT (Contributor)
Format: Article
Language:English
Published: Elsevier BV, 2020-07-31T13:28:44Z.
Subjects:
Article
Online Access:Get fulltext

Internet

Get fulltext

Similar Items