Structural basis for the altered PAM recognition by engineered CRISPR-Cpf1

Structural basis for the altered PAM recognition by engineered CRISPR-Cpf1

The RNA-guided Cpf1 nuclease cleaves double-stranded DNA targets complementary to the CRISPR RNA (crRNA), and it has been harnessed for genome editing technologies. Recently, Acidaminococcus sp. BV3L6 (AsCpf1) was engineered to recognize altered DNA sequences as the protospacer adjacent motif (PAM),...

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Bibliographic Details
Main Authors: Nishimasu, Hiroshi (Author), Yamano, Takashi (Author), Gao, Linyi (Author), Zhang, Feng (Author), Ishitani, Ryuichiro (Author), Nureki, Osamu (Author)
Other Authors: Broad Institute of MIT and Harvard (Contributor), McGovern Institute for Brain Research at MIT (Contributor), Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences (Contributor)
Format: Article
Language:English
Published: Elsevier BV, 2020-08-14T20:30:19Z.
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