High-Level Expression, Single-Step Immunoaffinity Purification and Characterization of Human Tetraspanin Membrane Protein CD81

High-Level Expression, Single-Step Immunoaffinity Purification and Characterization of Human Tetraspanin Membrane Protein CD81

The study of membrane protein structure and function requires their high-level expression and purification in fully functional form. We previously used a tetracycline-inducible stable mammalian cell line, HEK293S-TetR, for regulated high-level expression of G-protein coupled receptors. We here repor...

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Bibliographic Details
Main Authors: Khorana, H. Gobind (Contributor), Zhang, Shuguang (Contributor), Chelikani, Prashen (Contributor), Takayama, Hidehito (Contributor), Reeves, Philip J. (Author)
Other Authors: Massachusetts Institute of Technology. Center for Biomedical Engineering (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Chemistry (Contributor)
Format: Article
Language:English
Published: Public Library of Science, 2010-06-02T15:45:37Z.
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