Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR

Background: Accurate interpretation of quantitative PCR (qPCR) data requires normalization using constitutively expressed reference genes. Ribosomal RNA is often used as a reference gene for transcriptional studies in E. coli. However, the choice of reliable reference genes has not been systematical...

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Bibliographic Details
Main Authors: Zhou, Kang (Author), Zhou, Lihan (Author), Lim, Qing 'En (Author), Zou, Ruiyang (Author), Stephanopoulos, Gregory (Contributor), Too, Heng-Phon (Author)
Other Authors: Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor)
Format: Article
Language:English
Published: BioMed Central Ltd, 2011-06-23T17:03:13Z.
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