Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR
Background: Accurate interpretation of quantitative PCR (qPCR) data requires normalization using constitutively expressed reference genes. Ribosomal RNA is often used as a reference gene for transcriptional studies in E. coli. However, the choice of reliable reference genes has not been systematical...
Main Authors: | Zhou, Kang (Author), Zhou, Lihan (Author), Lim, Qing 'En (Author), Zou, Ruiyang (Author), Stephanopoulos, Gregory (Contributor), Too, Heng-Phon (Author) |
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Other Authors: | Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor) |
Format: | Article |
Language: | English |
Published: |
BioMed Central Ltd,
2011-06-23T17:03:13Z.
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Subjects: | |
Online Access: | Get fulltext |
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