Effect of photobleaching on calibration model development in biological Raman spectroscopy
A major challenge in performing quantitative biological studies using Raman spectroscopy lies in overcoming the influence of the dominant sample fluorescence background. Moreover, the prediction accuracy of a calibration model can be severely compromised by the quenching of the endogenous fluorophor...
Main Authors: | , , , |
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Other Authors: | , |
Format: | Article |
Language: | English |
Published: |
Society of Photo-optical Instrumentation Engineers (SPIE),
2011-07-28T15:46:43Z.
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Subjects: | |
Online Access: | Get fulltext |