Optimized Expression and Purification for High-Activity Preparations of Algal [FeFe]-Hydrogenase

Optimized Expression and Purification for High-Activity Preparations of Algal [FeFe]-Hydrogenase

Background: Recombinant expression and purification of metallo-enzymes, including hydrogenases, at high-yields is challenging due to complex, and enzyme specific, post-translational maturation processes. Low fidelities of maturation result in preparations containing a significant fraction of inactiv...

Full description

Bibliographic Details
Main Authors: Yacoby, Iftach (Contributor), Tegler, Lotta Tollstoy (Contributor), Pochekailov, Sergii (Contributor), Zhang, Shuguang (Contributor), King, Paul W. (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Media Laboratory (Contributor), Program in Media Arts and Sciences (Massachusetts Institute of Technology) (Contributor)
Format: Article
Language:English
Published: Public Library of Science, 2012-07-20T18:41:43Z.
Subjects:
Article
Online Access:Get fulltext

Internet

Get fulltext

Similar Items