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|a Choi, Kee-Hyun
|e author
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|a Massachusetts Institute of Technology. Department of Chemistry
|e contributor
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|a Licht, Stuart
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|a Choi, Kee-Hyun
|e contributor
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|a Licht, Stuart
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|a Licht, Stuart
|e author
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|a ATP-Sensitive Potassium Channels Exhibit Variance in the Number of Open Channels below the Limit Predicted for Identical and Independent Gating
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|b Public Library of Science,
|c 2012-07-23T18:16:53Z.
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|z Get fulltext
|u http://hdl.handle.net/1721.1/71759
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|a In small cells containing small numbers of ion channels, noise due to stochastic channel opening and closing can introduce a substantial level of variability into the cell's membrane potential. Negatively cooperative interactions that couple a channel's gating conformational change to the conformation of its neighbor(s) provide a potential mechanism for mitigating this variability, but such interactions have not previously been directly observed. Here we show that heterologously expressed ATP-sensitive potassium channels generate noise (i.e., variance in the number of open channels) below the level possible for identical and independent channels. Kinetic analysis with single-molecule resolution supports the interpretation that interchannel negative cooperativity (specifically, the presence of an open channel making a closed channel less likely to open) contributes to the decrease in noise. Functional coupling between channels may be important in modulating stochastic fluctuations in cellular signaling pathways.
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|a Arnold and Mabel Beckman Foundation
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|a Massachusetts Institute of Technology. Dept. of Chemistry
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|a en_US
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|a Article
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|t PLoS ONE
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