Methodological considerations for global analysis of cellular FLIM/FRET measurements

Methodological considerations for global analysis of cellular FLIM/FRET measurements

Global algorithms can improve the analysis of fluorescence energy transfer (FRET) measurement based on fluorescence lifetime microscopy. However, global analysis of FRET data is also susceptible to experimental artifacts. This work examines several common artifacts and suggests remedial experimental...

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Bibliographic Details
Main Authors: Rahim, Nur Aida Adbul (Contributor), Pelet, Serge (Contributor), Kamm, Roger Dale (Contributor), So, Peter T. C. (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Mechanical Engineering (Contributor)
Format: Article
Language:English
Published: SPIE, 2012-08-14T14:26:43Z.
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