Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator

Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator

We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to det...

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Bibliographic Details
Main Authors: Chen, Chia-Hung (Author), Sarkar, Aniruddh (Contributor), Song, Yong-Ak (Contributor), Miller, Miles Aaron (Contributor), Kim, Sung Jae (Contributor), Griffith, Linda G. (Contributor), Lauffenburger, Douglas A. (Contributor), Han, Jongyoon (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science (Contributor), Goyal, Chia-Hung (Contributor)
Format: Article
Language:English
Published: American Chemical Society (ACS), 2012-09-25T14:05:25Z.
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