Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator

We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to det...

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Bibliographic Details
Main Authors: Chen, Chia-Hung (Author), Sarkar, Aniruddh (Contributor), Song, Yong-Ak (Contributor), Miller, Miles Aaron (Contributor), Kim, Sung Jae (Contributor), Griffith, Linda G. (Contributor), Lauffenburger, Douglas A. (Contributor), Han, Jongyoon (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science (Contributor), Goyal, Chia-Hung (Contributor)
Format: Article
Language:English
Published: American Chemical Society (ACS), 2012-09-25T14:05:25Z.
Subjects:
Online Access:Get fulltext
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042 |a dc 
100 1 0 |a Chen, Chia-Hung  |e author 
100 1 0 |a Massachusetts Institute of Technology. Department of Biological Engineering  |e contributor 
100 1 0 |a Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science  |e contributor 
100 1 0 |a Goyal, Chia-Hung  |e contributor 
100 1 0 |a Sarkar, Aniruddh  |e contributor 
100 1 0 |a Song, Yong-Ak  |e contributor 
100 1 0 |a Miller, Miles Aaron  |e contributor 
100 1 0 |a Kim, Sung Jae  |e contributor 
100 1 0 |a Griffith, Linda G.  |e contributor 
100 1 0 |a Lauffenburger, Douglas A.  |e contributor 
100 1 0 |a Han, Jongyoon  |e contributor 
700 1 0 |a Sarkar, Aniruddh  |e author 
700 1 0 |a Song, Yong-Ak  |e author 
700 1 0 |a Miller, Miles Aaron  |e author 
700 1 0 |a Kim, Sung Jae  |e author 
700 1 0 |a Griffith, Linda G.  |e author 
700 1 0 |a Lauffenburger, Douglas A.  |e author 
700 1 0 |a Han, Jongyoon  |e author 
245 0 0 |a Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator 
260 |b American Chemical Society (ACS),   |c 2012-09-25T14:05:25Z. 
856 |z Get fulltext  |u http://hdl.handle.net/1721.1/73163 
520 |a We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (~10-fold) reduced the time required to complete the assay and the sample volume used. 
520 |a National Institutes of Health (U.S.) (Grant GM68762) 
520 |a National Institutes of Health (U.S.) (Grant U54-CA112967) 
520 |a National Institutes of Health (U.S.) (Grant R01-EB010246) 
520 |a National Institutes of Health (U.S.) (Grant R01-GM081336) 
520 |a National Science Foundation (U.S.) (Graduate Fellowship) 
520 |a United States. Defense Advanced Research Projects Agency (Cipher Program) 
546 |a en_US 
655 7 |a Article 
773 |t Journal of the American Chemical Society