High-throughput in vivo vertebrate screening

• Main Authors: Pardo-Martin, Carlos (Contributor), Chang, Tsung-Yao (Contributor), Koo, Bryan Kyo (Contributor), Gilleland, Cody Lee (Contributor), Wasserman, Steven Charles (Contributor), Yanik, Mehmet Fatih (Contributor)
• Other Authors: move to dc.description.sponsorship (Contributor), Harvard University- (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science (Contributor)
• Format: Article
• Language: English
• Published: Nature Publishing Group, 2012-10-04T16:15:36Z.
• Subjects: Article
• Online Access: Get fulltext

We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.
National Institutes of Health (U.S.) (Director's Innovator Award 1-DP2-OD002989-01)
David & Lucile Packard Foundation (Award in Science and Engineering)
Alfred P. Sloan Foundation (Award)
Broad Institute of MIT and Harvard (Sparc Grant)
National Science Foundation (U.S.) (Fellowship)
Foxconn (Sponsorship)