Rapid dissection and model-based optimization of inducible enhancers in human cells using a massively parallel reporter assay

Rapid dissection and model-based optimization of inducible enhancers in human cells using a massively parallel reporter assay

Learning to read and write the transcriptional regulatory code is of central importance to progress in genetic analysis and engineering. Here we describe a massively parallel reporter assay (MPRA) that facilitates the systematic dissection of transcriptional regulatory elements. In MPRA, microarray-...

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Bibliographic Details
Main Authors: Melnikov, Alexandre (Author), Murugan, Anand (Author), Zhang, Xiaolan (Author), Tesileanu, Tiberiu (Author), Wang, Li (Author), Rogov, Peter (Author), Feizi-Khankandi, Soheil (Contributor), Gnirke, Andreas (Author), Callan Jr, Curtis G. (Author), Kinney, Justin B. (Author), Kellis, Manolis (Contributor), Lander, Eric S. (Contributor), Mikkelsen, Tarjei Sigurd 1978- (Author)
Other Authors: Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science (Contributor)
Format: Article
Language:English
Published: Nature Publishing Group, 2012-10-18T18:32:29Z.
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