Microfluidic Preparative Free-Flow Isoelectric Focusing: System Optimization for Protein Complex Separation

Microfluidic Preparative Free-Flow Isoelectric Focusing: System Optimization for Protein Complex Separation

Isoelectric focusing (IEF) is the first step for two-dimensional (2D) gel electrophoresis and plays an important role in sample purification for proteomics. However, biases in protein size and pI resolution, as well as limitations in sample volume, gel capacity, sample loss, and experimental time, r...

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Bibliographic Details
Main Authors: Wen, Jian (Contributor), Wilker, Erik W. (Contributor), Yaffe, Michael B (Author), Jensen, Klavs F (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor), Koch Institute for Integrative Cancer Research at MIT (Contributor), Yaffe, Michael B. (Contributor), Jensen, Klavs F. (Contributor)
Format: Article
Language:English
Published: American Chemical Society (ACS), 2012-11-26T17:43:54Z.
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