Microfluidic Preparative Free-Flow Isoelectric Focusing: System Optimization for Protein Complex Separation
Isoelectric focusing (IEF) is the first step for two-dimensional (2D) gel electrophoresis and plays an important role in sample purification for proteomics. However, biases in protein size and pI resolution, as well as limitations in sample volume, gel capacity, sample loss, and experimental time, r...
Main Authors: | Wen, Jian (Contributor), Wilker, Erik W. (Contributor), Yaffe, Michael B (Author), Jensen, Klavs F (Author) |
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Other Authors: | Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor), Koch Institute for Integrative Cancer Research at MIT (Contributor), Yaffe, Michael B. (Contributor), Jensen, Klavs F. (Contributor) |
Format: | Article |
Language: | English |
Published: |
American Chemical Society (ACS),
2012-11-26T17:43:54Z.
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Subjects: | |
Online Access: | Get fulltext |
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