Nano-Layered Microneedles for Transcutaneous Delivery of Polymer Nanoparticles and Plasmid DNA

• Main Authors: DeMuth, Peter Charles (Contributor), Su, Xingfang (Contributor), Samuel, Raymond E. (Contributor), Hammond, Paula T (Author), Irvine, Darrell J (Author)
• Other Authors: Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor), Massachusetts Institute of Technology. Department of Materials Science and Engineering (Contributor), Ragon Institute of MGH, MIT and Harvard (Contributor), Koch Institute for Integrative Cancer Research at MIT (Contributor), Hammond, Paula T. (Contributor), Irvine, Darrell J. (Contributor)
• Format: Article
• Language: English
• Published: Wiley-Blackwell Publishers, 2013-06-26T16:33:34Z.
• Subjects: Article
• Online Access: Get fulltext

Multilayer-coated microneedles achieve transcutaneous delivery of plasmid DNA to the viable epidermis. Cy3-labeled plasmid DNA encoding luciferase (yellow) is deposited on biodegradable microneedle arrays through multilayer self-assembly and then delivered to the skin by microneedle application to achieve colocalization with Langerhans dendritic cells (MHC II-GFP-green).
United States. Dept. of Defense (DAAD19- 02-D-0002)
United States. Dept. of Defense (W911NF-07-D-0004)
Ragon Institute of MGH, MIT and Harvard
National Institutes of Health (U.S.) (NIH Biotechnology Training Program at MIT)
Howard Hughes Medical Institute (Investigator)