Intracellular Water Exchange for Measuring the Dry Mass, Water Mass and Changes in Chemical Composition of Living Cells

Intracellular Water Exchange for Measuring the Dry Mass, Water Mass and Changes in Chemical Composition of Living Cells

We present a method for direct non-optical quantification of dry mass, dry density and water mass of single living cells in suspension. Dry mass and dry density are obtained simultaneously by measuring a cell's buoyant mass sequentially in an H[subscript 2]O-based fluid and a D[subscript 2]O-ba...

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Main Authors: Cermak, Nathan (Contributor), Son, Sungmin (Contributor), Li, Yingzhong (Contributor), Higgins, John M. (Author), Chen, Jianzhu (Contributor), Grover, William H. (Contributor), Delgado, Francisco Feijo (Contributor), Hecht, Vivian Chaya (Contributor), Olcum, Selim A. (Author), Manalis, Scott R (Author), Knudsen, Scott (Author)
Other Authors: Massachusetts Institute of Technology. Computational and Systems Biology Program (Contributor), Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Koch Institute for Integrative Cancer Research at MIT (Contributor), Knudsen, Scott Michael (Contributor), Olcum, Selim (Contributor), Manalis, Scott R. (Contributor)
Format: Article
Language:English
Published: Public Library of Science, 2013-09-16T15:22:54Z.
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