Protein Thioester Synthesis Enabled by Sortase
Proteins containing a C-terminal thioester are important intermediates in semisynthesis. Currently there is one main method for the synthesis of protein thioesters that relies upon the use of engineered inteins. Here we report a simple strategy, utilizing sortase A, for routine preparation of recomb...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | English |
| Published: |
American Chemical Society,
2013-11-25T17:16:55Z.
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| Subjects: | |
| Online Access: | Get fulltext |