Thermal Stabilization of an Endoglucanase by Cyclization

An intein-driven protein splicing approach allowed for the covalent linkage between the N- and C-termini of a polypeptide chain to create circular variants of the endo-β-1,3-1,4-glucanase, LicA, from Bacillus licheniformis. Two circular variants, LicA-C1 and LicA-C2, which have connecting loops of 2...

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Bibliographic Details
Main Authors: Lieshout, Johan F. T. (Author), Vroom, Wietse (Author), Planas, Antoni (Author), Vos, Willem M. (Author), Oost, John (Author), Koutsopoulos, Sotirios (Author), Gutierrez, Odette N. Perez (Author), Kouusopoulos, Sotiros (Contributor)
Other Authors: Massachusetts Institute of Technology. Center for Biomedical Engineering (Contributor)
Format: Article
Language:English
Published: Springer-Verlag, 2013-12-06T15:30:50Z.
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