Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Ideal cell-free expression systems can theoretically emulate an in vivo cellular environment in a controlled in vitro platform. This is useful for expressing proteins and genetic circuits in a controlled manner as well as for providing a prototyping environment for synthetic biology. To achieve the...

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Main Authors: Sun, Zachary Z. (Author), Hayes, Clarmyra A. (Author), Shin, Jonghyeon (Contributor), Caschera, Filippo (Author), Murray, Richard M. (Author), Noireaux, Vincent (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Synthetic Biology Center (Contributor)
Format: Article
Language:English
Published: MyJoVE Corporation, 2014-09-16T20:01:57Z.
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