Somatic retrotransposition in human cancer revealed by whole-genome and exome sequencing

• Main Authors: Helman, Elena (Contributor), Lawrence, Michael S. (Author), Stewart, Chip (Author), Sougnez, Carrie (Author), Getz, Gad (Author), Meyerson, Matthew L. (Author)
• Other Authors: Harvard University- (Contributor), Meyerson, Matthew (Contributor)
• Format: Article
• Language: English
• Published: Cold Spring Harbor Laboratory Press, 2014-12-02T17:01:44Z.
• Subjects: Article
• Online Access: Get fulltext

 Retrotransposons constitute a major source of genetic variation, and somatic retrotransposon insertions have been reported in cancer. Here, we applied TranspoSeq, a computational framework that identifies retrotransposon insertions from sequencing data, to whole genomes from 200 tumor/normal pairs across 11 tumor types as part of The Cancer Genome Atlas (TCGA) Pan-Cancer Project. In addition to novel germline polymorphisms, we find 810 somatic retrotransposon insertions primarily in lung squamous, head and neck, colorectal, and endometrial carcinomas. Many somatic retrotransposon insertions occur in known cancer genes. We find that high somatic retrotransposition rates in tumors are associated with high rates of genomic rearrangement and somatic mutation. Finally, we developed TranspoSeq-Exome to interrogate an additional 767 tumor samples with hybrid-capture exome data and discovered 35 novel somatic retrotransposon insertions into exonic regions, including an insertion into an exon of the PTEN tumor suppressor gene. The results of this large-scale, comprehensive analysis of retrotransposon movement across tumor types suggest that somatic retrotransposon insertions may represent an important class of structural variation in cancer.