Genetic Screens in Human Cells Using the CRISPR-Cas9 System

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

The bacterial clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system for genome editing has greatly expanded the toolbox for mammalian genetics, enabling the rapid generation of isogenic cell lines and mice with modified alleles. Here, we describe a pooled, loss-of-function g...

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Bibliographic Details
Main Authors: Wang, Tim (Contributor), Wei, Jenny J. (Contributor), Sabatini, David (Author), Lander, Eric Steven (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biological Engineering (Contributor), Massachusetts Institute of Technology. Department of Biology (Contributor), Whitehead Institute for Biomedical Research (Contributor), Koch Institute for Integrative Cancer Research at MIT (Contributor), Sabatini, David M. (Contributor), Lander, Eric S. (Contributor)
Format: Article
Language:English
Published: American Association for the Advancement of Science (AAAS), 2015-04-17T18:00:32Z.
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