Structure-Function Studies of Serine Hydrolases: Synthesis of a Gene for α-Lytic Protease and the Purification and Characterization of a Mutant β-Lactamase
<p>The author has constructed a synthetic gene for α-lytic protease. Since the DNA sequence of the protein is not known, the gene was designed by using the reverse translation of α-lytic protease's amino acid sequence. Unique restriction sites are carefully sought in the degenerate DNA se...
Internet
https://thesis.library.caltech.edu/7523/5/Perez-dm-1988.pdfPerez, Dianne Marie (1988) Structure-Function Studies of Serine Hydrolases: Synthesis of a Gene for α-Lytic Protease and the Purification and Characterization of a Mutant β-Lactamase. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/brjj-5n58. https://resolver.caltech.edu/CaltechTHESIS:03182013-143408752 <https://resolver.caltech.edu/CaltechTHESIS:03182013-143408752>