Structure-Function Studies of Serine Hydrolases: Synthesis of a Gene for α-Lytic Protease and the Purification and Characterization of a Mutant β-Lactamase

<p>The author has constructed a synthetic gene for α-lytic protease. Since the DNA sequence of the protein is not known, the gene was designed by using the reverse translation of α-lytic protease's amino acid sequence. Unique restriction sites are carefully sought in the degenerate DNA se...

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Bibliographic Details
Main Author: Perez, Dianne Marie
Format: Others
Language:en
Published: 1988
Online Access:https://thesis.library.caltech.edu/7523/5/Perez-dm-1988.pdf
Perez, Dianne Marie (1988) Structure-Function Studies of Serine Hydrolases: Synthesis of a Gene for α-Lytic Protease and the Purification and Characterization of a Mutant β-Lactamase. Dissertation (Ph.D.), California Institute of Technology. doi:10.7907/brjj-5n58. https://resolver.caltech.edu/CaltechTHESIS:03182013-143408752 <https://resolver.caltech.edu/CaltechTHESIS:03182013-143408752>