Generation of human induced pluripotent stem cells using non-synthetic mRNA
Here we describe some of the crucial steps to generate induced pluripotent stemcells (iPSCs) usingmRNA transfection. Our approach uses a V. virus-derived capping enzyme instead of a cap-analog, ensuring 100% proper cap orientation for in vitro transcribedmRNA. V. virus\' 2′-O-Methyltransferase...
Main Authors: | , , , , , , , , |
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Other Authors: | |
Format: | Article |
Language: | English |
Published: |
Universitätsbibliothek Leipzig
2016
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Subjects: | |
Online Access: | http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-205889 http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-205889 http://www.qucosa.de/fileadmin/data/qucosa/documents/20588/OAP-2016-105_stolzing_1-s2.0-S187350611630006X-main.pdf |