Optimization of Recombination Methods and Expanding the Utility of Penicillin G Acylase

Protein engineering can be performed by combinatorial techniques (directed evolution) and data-driven methods using machine-learning algorithms. The main characteristic of directed evolution (DE) is the application of an effective and efficient screen or selection on a diverse mutant library. As it...

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Bibliographic Details
Main Author: Loo, Bernard Liat Wen
Published: Georgia Institute of Technology 2008
Subjects:
Online Access:http://hdl.handle.net/1853/19728