Development of a novel dehydrogenase and a stable cofactor regeneration system
The first goal of this work focused on the development of an amine dehydrogenase (AmDH) from a leucine dehydrogenase using site-directed mutagenesis. We aimed at reductively aminating a prochiral ketone to a chiral amine by using leucine dehydrogenase (LeuDH) as a starting template. This initial w...
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Georgia Institute of Technology
2010
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| Online Access: | http://hdl.handle.net/1853/31685 |