Structural investigation of gelsolin superfamily members
We expressed three recombinant proteins in E. coli and purified them for analysis. Human gelsolin fragment G2-G3 retains the ability of intact gelsolin to bind to and co-sediment with filamentous actin. Tryptophan fluorescence intensity from G2-G3 is Ca²⁺-sensitive, increasing with Ca²⁺ concentratio...
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| Language: | English |
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University of British Columbia
2012
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| Online Access: | http://hdl.handle.net/2429/40996 |