Structural examination of voltage gated potassium channels by voltage clamp fluorometry
Voltage clamp fluorometry (VCF) was first developed in the mid 1990s by Isacoff and his colleagues. In this approach fluorophores are attached to substituted cysteine residues that are engineered by site-directed mutagenesis. Changes in the dielectric environment of the fluorophore report local tran...
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| Language: | English |
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University of British Columbia
2008
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| Online Access: | http://hdl.handle.net/2429/1709 |