Conversion of subtilisin E to thiolsubtilisin by site-directed mutagenesis
This thesis presents a procedure of site specific mutagenesis of subtilisin E for reducing its proteolytic activity while retaining its ligase activity in peptide synthesis. A host-vector system was chosen to conduct the DNA manipulation and the gene expression. Subtilisin B gene (aprE) cloned on...
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| Language: | English |
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2009
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| Online Access: | http://hdl.handle.net/2429/5125 |