A puhA gene deletion and plasmid complementation system for facile site directed mutagenesis studies of the reaction center H protein of Rhodobacter Sphaeroides
The development of a Rhodobacter sphaeroides deletion/plasmid complementation system of the puhA gene (which encodes the reaction center [RC] heavy [H] subunit) for expression of site directed mutants of the RC H protein is described. The mutant strain ΔPUHA was constructed by introduction of a...
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| Language: | English |
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2009
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| Online Access: | http://hdl.handle.net/2429/6516 |