Expression of Biotinylated Multivalent Peptide Antigens in Bacteria for Rapid and Effective Generation of Single Domain Antibodies from Phage-displayed Antibody Libraries
In the present study, two insulin-like growth factor-binding protein 7 (IGFBP7) C-terminal-peptides were expressed as fusion proteins to bacterial verotoxin pentamerization domain as shown by Western blotting, ELISA and mass spectroscopy. Both in vivo-biotinylated recombinant products were purified...
| Main Author: | |
|---|---|
| Language: | en |
| Published: |
2012
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/10393/23523 |