Identification of Ryanodine Receptor 1 (RyR1) Interacting Protein Partners Using Liquid Chromatography and Mass Spectrometry

Ryanodine receptor 1 (RyR1) is a homotetrameric calcium channel located in the sarcoplasmic reticulum (SR) of skeletal muscle. We employed metal affinity chromatography followed by liquid chromatography mass spectrometry from HEK-293 cells to purify affinity tagged cytosolic RyR1, with interacting p...

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Bibliographic Details
Main Author: Ryan, Timothy
Other Authors: Gramolini, Anthony
Language:en_ca
Published: 2010
Subjects:
Online Access:http://hdl.handle.net/1807/25911