Simultaneous clarification and purification of recombinant penicillin G acylase using tangential flow filtration anion-exchange membrane chromatography

Downstream purification often represents the most cost-intensive step in the manufacturing of recombinant proteins. Conventional purification processes are lengthy, technically complicated, product specific and time-consuming. To address this issue, herein we develop a one step purification system t...

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Bibliographic Details
Main Author: Orr, Valerie
Language:en
Published: 2012
Subjects:
Online Access:http://hdl.handle.net/10012/6601