| Summary: | The serum hexosamine and protein contents of avian, bovine, porcine, rodent (rat) and human subjects were estimated. Significant species and sex differences were detected. Maturation brought a large increase of serum hexosamine in female fowls and pigs, a small increase in cows, and no increase in humans. The effects of pregnancy in cows and exercise and diet in rats were also studied. In another study the effects of gonadal hormones, administered to immature fowl, on the levels of serum hexosamine, protein, glucuronic acid, sugar and calcium were investigated. Hydrolysis of serum with a polystyrene resin gave higher estimates of hexosamine content than hydrolysis or serum with 4 N hydrochloric acid and subsequent chromatography. A reliable estimate of the glucuronic acid content ofserum could not be obtained by a direct determination, but a relatively reliable estimate may be obtained after resin hydrolysis of serum and purification of hydrolyzate by passing it through cationic and anionic exchange resin columns.
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