Genetic and biochemical characterization of dihydrolipoamide dehydrogenases (LPD) in Sinorhizobium meliloti

To investigate the functionality of the Sinorhizobium meliloti dihydrolipoamide dehydrogenases, the lpdA1, lpdA2, and lpdA3 genes were mutated through the site-directed, single cross-over recombination, using modified pVIK112 suicide plasmids. The lpdA1 mutant failed to grow on pyruvate, and was si...

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Bibliographic Details
Main Author: Babic, Branislav
Other Authors: Brian T Driscoll (Supervisor)
Format: Others
Language:en
Published: McGill University 2011
Subjects:
Online Access:http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=96895