The Role of Perturbation on Biochemical Systems: Salt, PH, and Microgravity Effects on Protein-Ligand and Small Molecule Interactions

DNA binding by the type I DNA polymerases from Escherichia coli (Klenow) and Thermus aquaticus (Klentaq) has been examined under varying ionic conditions. Klenow and Klentaq both show increased affinity for DNA substrate in the presence of KAcetate over KCl. Ionic linkage relationships were determi...

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Bibliographic Details
Main Author: Thompson, Gregory S.
Other Authors: Marcia Newcomer
Format: Others
Language:en
Published: LSU 2005
Subjects:
Online Access:http://etd.lsu.edu/docs/available/etd-04072005-110000/