Engineering the Escherichia coli NikR Transcription Factor via Mutations in the α3 Helix
Escherichia coli NikR is a metal-responsive transcription factor that controls nickel uptake by regulating expression of a nickel-specific transporter, NikABCDE. NikR contains a high-affinity nickel-binding site, and nickel must occupy this site in order to activate DNA binding. Stoichiometric amoun...
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| Language: | en_ca |
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2014
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| Online Access: | http://hdl.handle.net/1807/65590 |