The effects of using different cereals as carbohydrate sources on oxidative stress、glycation and lipid metabolism in diabetic rats

• Main Authors: Shi-Hong Chen, 陳信宏
• Other Authors: Hsing-Hsien Cheng
• Format: Others
• Language: zh-TW
• Published: 2000
• Online Access: http://ndltd.ncl.edu.tw/handle/21116027111193430834

碩士 === 台北醫學院 === 保健營養學研究所 === 88 === Abstract Oxidative stress, glycation and lipid malmetabolism have been proposed to be linked and be increasing to tissue damage and the development of pathophysiology in diabetic patients. The purposed to evaluate the effects of using different cereals as carbohydrate source on oxidative status, protein glycation and lipid metabolism in diabetic rats. Fifty four male adult Wistar rats (b.w eq. 250-280g) were divided into six groups: two control groups were AIN-76 diet, with or without 180 °C precooked corn starch, C and R groups respectivelly. Another 4 groups were matched with wheat flour (H group) and different variety rices (I group: Indica rice, J group: Japonica rice, W group: Waxy rice ). Diabetic rats were induced by injecting with 45 mg/kg/day streptozotocin in citrate buffer 3 days, continuously. While the blood sugar over 270 mg/dL (about 15 mmol/L) which was defined as the diabetic rats, and then feeding the different experimental diets. Four weeks later, the diabetic rats were sacrificed and sampled. The indicator of oxidative status and protein glycation were individually evaluated the activity of antioxidant enzyme/MDA (malondialdehyde) and the HbA1c/fructosamine. In addition, we also evaluated the plasma TRAP (total radicals-trapping antioxidant parameter) to elucidate the plasma antioxidant capacity of diabetic rats. Resulting, there is no significant difference between the experimental groups on HbA1c/fructosamine. The liver SOD (superoxide dismutase) activity is higher (22.13 ± 2.83 U/mg protein, p  0.05), and the MDA is lower (0.07 ± 0.01 nmol/ mg protein, p  0.05) in J group. The kidney SOD activity is higher (13.65 ± 2.47 U/mg protein, p  0.05), the plasma TRAP also the J group is the highest (121.49 ± 32.36 M, p  0.05). The cholesterol level in liver, is the I group lower (0.39 ± 0.05 mmol/L, p  0.05). Concluding, the Japonica rice is good to reduce oxidative stress and the Indica rice lowers the liver cholesterol level in diabetic rats. key word: diabetic rats, antioxidant system, oxidative stress, glycation.