The investingation of generation of giant protoplasts of Escherichia coli and the applied of giant protoplasts

碩士 === 國立成功大學 === 化學工程學系碩博士班 === 90 === The present research used Lysozyme and EDTA to produce the protoplasts of Escherichia coli JM109 and incubated protoplasts in medium to form giant protoplasts. The addition of EDTA (1%, w/v) in SP buffer could make high degree of cell lysis and low concentrat...

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Bibliographic Details
Main Authors: Meng-Chuen Wu, 吳孟純
Other Authors: Chu-Yuan Cheng
Format: Others
Language:zh-TW
Published: 2002
Online Access:http://ndltd.ncl.edu.tw/handle/4km5hn