Purification and Characterization of Histidine Tagged Vacuolar H+ -pyrophosphatase Expressed in Yeast
碩士 === 國立清華大學 === 生命科學系 === 90 === H+-PPase (E.C.3.6.1.1) existed primarily in higher plants and several bacteria and requires PPi as energy source, supplying energy to the secondary active transport system. Fusion proteins containing a string of his-tag made it easy to be purified from...
Main Authors: | , |
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Other Authors: | |
Format: | Others |
Language: | zh-TW |
Published: |
2002
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Online Access: | http://ndltd.ncl.edu.tw/handle/90239210369255337647 |