| Summary: | 碩士 === 高雄醫學大學 === 藥學研究所碩士班 === 92 === The purpose of this study was to use HPLC analysis method to qualify 13 compounds in herbs and products of San-huang-xie-xin-tang and to build the fingerprints of San-huang-xie-xin-tang. Then we try to evaluate the interaction in vitro. Such procedures can make us evaluate the quality control and the active components pharmacokinetics of San-huang-xie-xin-tang.
We made single herb powder, single herb blank powder, and San
-huang-xie-xin-tang standard powder because we would like to know the herb origins of all compounds and the difference of pharmacokinetics in single herb and San-huang-xie-xin-tang.
In the study, one sample needs 60 minutes to analyze. We found baicalin percentage in Scutellariae radix was the most when we analyzed all samples. The fingerprint of Scutellariae radix was as the same as fingerprint of San-huang-xie-xin-tang almost. We also built the San-huang
-xie-xin-tang fingerprint. In interaction aspect, Coptidis rhizome with Rhei rhizome and Coptidis rhizome with Scutellariae radix made precipitate when decocting together.
We could analyze 6 compounds in rat plasma when we I.P. San
-huang-xie-xin-tang and single herb. According to the results of analysis in vivo, We found combination of herbs could make compound release and metabolize more slowly.
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